Phospholipase D (PLD) is widely distributed in mammalian cells, where it is regulated by a variety of extracellular signals. Its major substrate is phosphatidylcholine (PC), which is hydrolyzed to phosphatidic acid (PA) and choline. The PLD-catalysed PC hydrolysis is believed to be an important mechanism of signal transduction in cells. More and more lines of evidence indicate that PLD is tightly involved in inflammation. This review introduces the recent advances of PLD in respiratory burst, degranulation and arachidonic acid release.