[Culture and identify the endothelial cells of human umbilical vein].
Copyright policy )[Culture and identify the endothelial cells of human umbilical vein].
In order to improve the routine culture method for vascular endothelial cells, endothelial cells of vein from newborn umbilical cord were successfully isolated with combined using 0.1% collagenase and 0.25% trypsin (1:1). The endothelial cells were further abrated by mechanical method. The composition of culture medium was simplified by not adding vascular endothelial cell growth factor and heparin. The cultured cells were verified as vascular endothelial cells by morphology, ultramicromorphology and immunohistochemistry.
- Sichuan University China (People's Republic of)
- West China Medical Center of Sichuan University China (People's Republic of)
Umbilical Veins, Pregnancy, Cell Culture Techniques, Infant, Newborn, Humans, Female, Cell Separation, Endothelium, Vascular
Umbilical Veins, Pregnancy, Cell Culture Techniques, Infant, Newborn, Humans, Female, Cell Separation, Endothelium, Vascular
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