
Se desarrolló un protocolo exitoso para la propagación en masa de Lawsonia inermis Linn., una planta medicinal importante. Se indujeron múltiples tallos en meristemos apicales y axilares derivados de explantes maduros de L. inermis en medio de Murashige y Skoog (1962) suplementado con 0.25 mg/l 6-benzylaminopurina (BA), 0.25 mg/l quinetina (Kn), 0.5 mg/l ácido ascórbico y 3 % (w/v) sucrosa. La tasa de multiplicación fue más alta cuando los cultivos fueron incubados bajo luz continua que bajo el fotoperíodo de 14 hr. El enraizamiento se logró al transferir los microtallos al medio basal, semi-sólido MS suplementado con 0.25 mg/l de ácido indol-3-butírico (IBA) luego de 10 días de cultivo. Las plántulas micropropagadas fueron aclimatizadas y cultivadas con éxito en el suelo.
A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3 % (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil.
Lythraceae, micropropagation, shoot multiplication, Plants, Medicinal, QH301-705.5, Acclimatization, Reproduction, in vitro, Culture Media, In vitro, Biology (General), Plant Shoots, medicinal plants
Lythraceae, micropropagation, shoot multiplication, Plants, Medicinal, QH301-705.5, Acclimatization, Reproduction, in vitro, Culture Media, In vitro, Biology (General), Plant Shoots, medicinal plants
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