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Revista de Biología Tropical
Article . 2001
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In vitro micropropagation of Lawsonia inermis (Lythraceae).

Authors: Rout, G.R.; Das, G.; Samantaray, S.; Das, P.;

In vitro micropropagation of Lawsonia inermis (Lythraceae).

Abstract

Se desarrolló un protocolo exitoso para la propagación en masa de Lawsonia inermis Linn., una planta medicinal importante. Se indujeron múltiples tallos en meristemos apicales y axilares derivados de explantes maduros de L. inermis en medio de Murashige y Skoog (1962) suplementado con 0.25 mg/l 6-benzylaminopurina (BA), 0.25 mg/l quinetina (Kn), 0.5 mg/l ácido ascórbico y 3 % (w/v) sucrosa. La tasa de multiplicación fue más alta cuando los cultivos fueron incubados bajo luz continua que bajo el fotoperíodo de 14 hr. El enraizamiento se logró al transferir los microtallos al medio basal, semi-sólido MS suplementado con 0.25 mg/l de ácido indol-3-butírico (IBA) luego de 10 días de cultivo. Las plántulas micropropagadas fueron aclimatizadas y cultivadas con éxito en el suelo.

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3 % (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil.

Related Organizations
Keywords

Lythraceae, micropropagation, shoot multiplication, Plants, Medicinal, QH301-705.5, Acclimatization, Reproduction, in vitro, Culture Media, In vitro, Biology (General), Plant Shoots, medicinal plants

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Average
Average
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gold