[Nested coamplification polymerase chain reaction].
Copyright policy )[Nested coamplification polymerase chain reaction].
To develop a simple and reliable method for intensifying the hybridization signals of gene chips.The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
- Sichuan University China (People's Republic of)
Base Sequence, Microscopy, Fluorescence, Molecular Sequence Data, Nucleic Acid Hybridization, Reproducibility of Results, DNA Probes, Polymerase Chain Reaction, Edetic Acid, Fluorescent Dyes
Base Sequence, Microscopy, Fluorescence, Molecular Sequence Data, Nucleic Acid Hybridization, Reproducibility of Results, DNA Probes, Polymerase Chain Reaction, Edetic Acid, Fluorescent Dyes
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