This report describes a noninvasive method for direct laryngoscopy in mice as small as 20 g by using commercially available equipment. A pediatric otoscope is inserted into the mouth of an anesthetized mouse to provide a clear view of the vocal cords. The procedure takes less than a minute and has been successful without complications in the 65 mice studied to date. The technique can be used to instill test substances into the lungs or to perform oral endotracheal intubation for resuscitation, airway support, or controlled ventilation.