Comparative studies concerning detection of histone-type and protamine-type proteins were carried out on Chara species (C. vulgaris, C. tomentosa). Analysis of antheridia during spermiogenesis (stages I-X) of both Charta species showed very similar staining patterns obtained after reactions revealing the examined proteins. Cytochemical studies showed a replacement of lysine-rich histone proteins by more basic arginine-rich ones during medium spermiogenesis (st. VI-VIII) in two Charta species, while late spermiogenesis (st. IX) and mature spermatozoids (st. X) were characterised by the presence of protamine-like proteins only.