Lentil is an important food legume crop that is very recalcitrant to shoot regeneration and rooting under in vitro conditions. This study aimed to develop efficient and reliable protocol for in vitro plant regeneration. The reulsts of this study showed that In this context, 16 Turkish lentil cultivars were shoot tip, stem , hypocotyl, cotyledon and root as explants. The MS medium containg 4 mg/l BAP induced maximum number (8.25) of shoots per shoot tip explant of cv. Yesil 21. Whereaas, maximum number (28.33) of shoots per cotyledon explant of cv. Ozbek was induced on MS medium containing 1 mg/l IBA. However,IBA derived shoots were easy to root on MS medium containing 1.87 mg/l NAA. The regenerated explants were transferred to greenhouse for acclimatisation, flowering and seed set. It was concluded that in vitro shoot regeneration of lentils (Lens culinaris Medikus) was strongly influenced by the type of explant, plant growth regulator concentration and combinations. © 2014 Elsevier B.V., All rights reserved.