Investigation of the determinative state of the mouse inner cell mass. I. Aggregation of isolated inner cell masses with morulae.
Copyright policy )Investigation of the determinative state of the mouse inner cell mass. I. Aggregation of isolated inner cell masses with morulae.
Inner cell masses (ICMs) were dissected from 3 1/2- and 4 1/2-day blastocysts and cultured in contact with 2 1/2-day morulae. Blastocysts and morulae were homozygous for different electrophoretic variants of the enzyme glucose phosphate isomerase (GPI). Aggregation of ICMs and morulae was observed, and such aggregates were able to form blastocysts in vitro and morphologically normal foetuses in utero. GPI analysis of these conceptuses revealed that most were chimaeric. However, donor ICM-type isozyme was only detected in the embryonic and extra-embryonic fractions of the chimaeras and never in the trophoblastic fraction. Thus, ICM cells appear unable to form trophoblast derivatives even when exposed to 'outside' conditions as experienced by developing trophoblast cells. This is evidence that ICM cells, although not overtly differentiated, are determined by 3 1/2 days.
- Jackson Laboratory United States
Chimera, Glucose-6-Phosphate Isomerase, Mitosis, Mice, Inbred Strains, Embryo Transfer, Genes: Gpi-1 - Alucose phosphate isomerase-1 (all), Trophoblasts, Mice, Blastocyst, 900, Embryology:, Morphology:, Animals, Transplantation, Homologous, Female, Embryo Implantation, Cell Aggregation
Chimera, Glucose-6-Phosphate Isomerase, Mitosis, Mice, Inbred Strains, Embryo Transfer, Genes: Gpi-1 - Alucose phosphate isomerase-1 (all), Trophoblasts, Mice, Blastocyst, 900, Embryology:, Morphology:, Animals, Transplantation, Homologous, Female, Embryo Implantation, Cell Aggregation
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).57 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!