Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.
Copyright policy )Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.
We have purified the yeast U5 and U6 pre-mRNA splicing small nuclear ribonucleoproteins (snRNPs) by affinity chromatography and analyzed the associated polypeptides by mass spectrometry. The yeast U5 snRNP is composed of the two variants of U5 snRNA, six U5-specific proteins and the 7 proteins of the canonical Sm core. The U6 snRNP is composed of the U6 snRNA, Prp24, and the 7 Sm-Like (LSM) proteins. Surprisingly, the yeast DEAD-box helicase-like protein Prp28 is stably associated with the U5 snRNP, yet is absent from the purified U4/U6 x U5 snRNP. A novel yeast U5 and four novel yeast U4/U6 x U5 snRNP polypeptides were characterized by genetic and biochemical means to demonstrate their involvement in the pre-mRNA splicing reaction. We also show that, unlike the human tri-snRNP, the yeast tri-snRNP dissociated upon addition of ATP or dATP.
- California Institute of Technology United States
570, Saccharomyces cerevisiae Proteins, Ribonucleoprotein, U4-U6 Small Nuclear, RNA Splicing, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, Fungal Proteins, affinity chromatography, Adenosine Triphosphate, Deoxyadenine Nucleotides, RNA Precursors, Animals, Humans, Amino Acid Sequence, Ribonucleoprotein, U5 Small Nuclear, mass spectrometry, Sequence Homology, Amino Acid, Ribonucleoproteins, Small Nuclear, Cold Temperature, Eukaryotic Cells, Phenotype, spliceosome assembly, Gene Targeting
570, Saccharomyces cerevisiae Proteins, Ribonucleoprotein, U4-U6 Small Nuclear, RNA Splicing, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, Fungal Proteins, affinity chromatography, Adenosine Triphosphate, Deoxyadenine Nucleotides, RNA Precursors, Animals, Humans, Amino Acid Sequence, Ribonucleoprotein, U5 Small Nuclear, mass spectrometry, Sequence Homology, Amino Acid, Ribonucleoproteins, Small Nuclear, Cold Temperature, Eukaryotic Cells, Phenotype, spliceosome assembly, Gene Targeting
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