To investigate the chemical constituents of Tetrastigma hemsleyanum.Compounds were separated by rechromatography on silica gel from the chloroform solubles of its ethanol extracts, and the structures were determined by spectral analysis and chemical evidence.Three compounds were isolated and elucidated as 6'-O-benzoyldaucosterol, daucosterol and beta-sitosterol.All the three compounds were isolated from this plant for the first time, of which 6'-O-benzoyldaucosterol is a new natural product.