Tuber borchii Vittad., commonly called bianchetto (whitish truffle), is an edible truffle with excellent culinary qualities. However, bianchetto is often regarded as one of the lesser truffles because it is often sold mixed with morphologically similar species in the Puberulum clade (Jeandroz et al., 2008) such as Tuber maculatum Vittad., Tuber dryophilum Tul. & C. Tul. and Tuber puberulum Berk. & Broome which have very poor culinary characteristics. In this paper we define the characteristics of the commercial species T. borchii describing its morphological and genetic characteristics.