Feline panleukopenia is a viral disease known since the beginning of the 20th century that occurs in cats, causing severe leukopenia, gastro-enteritis and nervous signs. Feline panleukopenia is caused by feline panleukopenia virus (FPV), a DNA virus belong to the family Parvoviridae, genus Parvovirus, subgroup of feline parvovirus. Also the new antigenic variants of canine parvovirus (CPV) type 2a-2b-2c have gained the feline host range and can infect, replicate and cause disease in cats with clinical signs similar to feline panleukopenia. In this report, we performed a molecular study on feline parvovirus strains isolated from domestic cats to investigate which parvoviruses strains are spread in the feline population and to monitor the evolution of FPV in Italy. Twenty-one viral isolates from clinical samples of diseased cats collected between 2000-2007 were examined. Phylogenetic analysis of the VP2 gene sequences was performed using the PHYLIP version 3.6 and TREE PUZZLE version 5.0 softwares. The reliability of the phylogenetic trees were assessed by bootstrap resampling (1000 datasets). Nineteen strains were found to be FPV and 2 strains were CPV-2. These CPV-2 strains show high genetic complexity with the presence of more variants in the same animal. Analysis of the VP2 protein of Italian FPV strains did not show specific amino acid changes in antigenic epitopes so we did not observe new variants for FPV. The phylogenetic trees constructed with VP2 sequences of reference strains show the Italian FPV strains clustering with the other FPV strains except for the more recent isolates that form a separate virus lineage.