Introduction -The feline coronaviruses (FCoVs), single-stranded RNA viruses beloging to the Coronaviridae family, cause an inapparent or mild enteric infection but also cause a rare, fatal immune-mediated disease, feline infectious peritonitis (FIP). FCoVs are ubiquitous viruses with a prevalence of 55-60% above all where large number of cats are housed closely, but FIP is uncommon manifestation of this infection. The aim of this work was to set up a Real Time PCR for the detection and quantification of FCoVs. Methods - Viral RNA of strain 420/02 liver was extracted from tissue and a fragment of ORF7b was amplified by RT-PCR One Tube. The PCR product of 102 bp has been cloned and serial diluitions of the recombinant plasmid were tested with a rotor-gene SYBER Green Real Time system to obtain a standard curve. Results - The Real Time assay was able to detect 10-20 copies of DNA/_l with a reaction efficiency from 0.766 to 0.786 and a R^2 Value over 0.99. Discussion - Real-Time PCR is resulted a sensitive and fast technique and its application to cats' organs or feces and comparation with other diagnostic tests are necessary to evaluate sensitivity and diagnostic employ.