Cow’s milk protein allergy (CMPA) is an abnormal immunological reaction to cow milk proteins, which result in IgE-mediated reactions. The therapeutic strategy of CMPA is represented by the total elimination of milk or by the administration of cow’s milk substitutes (1). For this reason the use of milk from other mammalian species was tested. Among them, donkey’s milk (DM) proved to be the best alternative in feeding infants affected by CMPA, since its chemical composition is comparable to human milk (1). In this work an “in vitro” study was performed in order to analyze the IgE reactivity to milk protein allergens from cow, donkey and goat by immunoblotting experiments using sera from milk-allergic and non allergic adult volunteers, with the aim of verifying the hypoallergenic property of donkey milk.