Brucella ovis was isolated from two rams, belonging to a flock located in Bergamo province, affected by unilateral epididymitis. B. ovis was recovered both from the epididymis and testicle with apparent lesions and from the apparently non affected organs. The microganism was also recovered from prostate from prostate and bulbourethral glands of both rams. The results of bacteriological investigation were confirmed by histopathological and immuno-histochemical examinations.