In this paper an extraction procedure is presented for sulphonamides (SAs) in animal tissues(raw meat and meat-based baby food), based on matrix solid phase dispersion (MSPD).Determination was carried out using liquid chromatography coupled with tandem mass spectrometry.In complex matrices extraction is usually the critical step of analysis: several procedureswere employed for the recovery of SAs in animal tissues, but often they entail time andsolvent-consuming processes. MSPD has demonstrated itself to be a very efficient extractiontechnique for matrices such as animal tissues, with simplified and shortened procedures, andvery low solvent consumption. This procedure allowed to obtain quantitative recovery, highselectivity improved by a cool eluant, and easy application. Good recoveries are accompaniedby low RSDs and not a significative matrix effect. The MSPD procedure, coupled with LC-MSMSanalysis, provides very interesting LODs, due also to the concentration step. Validation iscarried out following the EU guidelines for confirmation methods (EU Dec. 657/02).[...]