Determination of hemostatic proteins is important branch of diagnostic medicine. New direction in the use of micromycetes enzymes is the development of diagnosticums for determining the key components of the hemostasis system, that is cheaper and more available than now used proteases isolated from the human bloodstream, recombinant proteins or native proteinases, derived from snake venom. The aim of this work was selection of optimal concentrations range of extracellular protease-activator of micromycete Aspergillus ochraceus and A. flavus O-1. These proteases have protein C, factor X, t-PA, fibrin, collagen, elastin, plasmin and thrombin activator activity.