Grapevine leafroll disease occurs in all major grape-growing areas. Several Grapevine leafroll associated viruses (GLRaVs) have been shown to be trasmitted by mealybugs. Nine mealybugs have been reported as vectors of GLRaV-3 although detailed characterization of GLRaV-1 vectors trasmission is lacking. The presence of GLRaV-1 and Planococcus citri in italian vineyards made the study of transmission necessary. Acquisition and transmission of GLRaV-1 by all instarts of Planococcus citri repreent the object of this research. The present study showed Pl. citri as an effective vector of GLRaV-1, infact all instarts were able to acquire and transmit virus. Moreover, P. citri third instar showed to lose their infection status after molting.