[Identification of notoginseng(Panax notoginseng) and its adulterants using DNA sequencing].
Copyright policy )[Identification of notoginseng(Panax notoginseng) and its adulterants using DNA sequencing].
To analyze the nuclear ribosomal RNA small subunit (18S rRNA) and chloroplast matK gene sequence of notoginseng (Panax notoginseng) in order to provide molecular evidence for its genuine origin identification.To sequence 18S rRNA and matK genes of Panax notoginseng and its four adulterants such as P. japonicus, Curcuma phaeocaulis, C. wenyujin, C. kwangsiensis using PCR direct sequencing and to detect their variation of sequences.The sequence length of notoginseng and its adulterants is 1809-1811 bp for 18S rRNA gene and 1259-1548 bp for matK gene, respectively. Multiple sequence alignment shows that there are much sequence variation between notoginseng and its adulterants.DNA sequencing is an accurate and reliable method in origin identification of the genuine notoginseng.
- Institute of Chinese Materia Medica China (People's Republic of)
Quality Control, Base Sequence, Plant Stems, Molecular Sequence Data, Panax, Sequence Homology, Sequence Analysis, DNA, Protein-Tyrosine Kinases, Plant Roots, Zingiberaceae, RNA, Ribosomal, 18S, Amino Acid Sequence, Drugs, Chinese Herbal
Quality Control, Base Sequence, Plant Stems, Molecular Sequence Data, Panax, Sequence Homology, Sequence Analysis, DNA, Protein-Tyrosine Kinases, Plant Roots, Zingiberaceae, RNA, Ribosomal, 18S, Amino Acid Sequence, Drugs, Chinese Herbal
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