The effect of simulated weightlessness on thermoregulation was studied in 5 subjects. The experiment consisted of 3d baseline measurements, 7d head-down bed rest and 2d recovery. Circadian rhythm was assessed by continuous measurements of rectal temperature and skin temperature with 2h intervals. Heat Stress Protein 70 (HSP70) was measured by Western-blot Dot method and the facial surface temperature distribution was measured by HR-2 infrared thermography. The results showed that rectal temperature keeps the wake-sleep variation, but the circadian rhythm changed during bed rest, and the change of rectum temperature rhythm appeared mainly in the early-days of bed rest; HSP70 and facial surface temperature increased during bed rest.