An effective SPR nanosensor based on core-shell nanoparticles (Ag@AuNPs) incorporated hexagonal boron nitride (HBN) nanosheets and molecularly imprinted polymer (MIP) was presented for etoposide (ETO) detection. Scanning electron microscope (SEM), transmission electron microscope (TEM), x-ray diffraction (XRD) method, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) methods were utilized for all characterizations of nanomaterials and polymer surfaces. ETO imprinted SPR nanosensor based on Ag@AuNPs-HBN nanocomposite was developed in the presence of poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) [p(HEMA-MAGA)]. The results of the study have revealed that 0.001-1.00 ng mL(-1) (1.70 x 10(-12)-1.70 x 10(-9) M) and 0.00025 ng mL(-1) (4.25 x 10(-13) M) were found as the linearity range and the detection limit (LOD). Furthermore, the prepared SPR nanosensor was examined in terms of stability, repeatability and selectivity. Finally, the imprinted SPR nanosensor was applied to the urine samples having high recovery. C1 [Ozkan, Abdullah] Iskenderun Tech Univ, Fac Engn & Nat Sci, Dept Petr & Nat Gas, Antakya, Turkey. [Atar, Necip] Pamukkale Univ, Fac Engn, Dept Chem Engn, Denizli, Turkey. [Yola, Mehmet Lutfi] Iskenderun Tech Univ, Fac Engn & Nat Sci, Dept Biomed Engn, Antakya, Turkey.