
handle: 11468/25520
Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic mass-es were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with 0.5-4 mg/l benzylaminop-urine. After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somat-ic embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets.
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