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Propagación in vitro de cedro (cedrela odorata L.) vía organogenésis

Authors: Huaman Cachique, Guadalupe Ximena;

Propagación in vitro de cedro (cedrela odorata L.) vía organogenésis

Abstract

Desde muchas décadas, especies forestales vienen recobrando gran importancia aditiva. Actividades de sobre explotación forestal selectiva practicada durante los anos 80 en regiones amazónicas de América del Sur han generado gran disturbio sobre su distribución poblacional, colocando en riesgo hábitats o Jugares de origen. El Cedro (Cedrela odorata L.), una de las especies explotadas, paso a ser considerada desde 1981 como una especie prioritaria para conservación (Lahera, 1995); desde ese entonces fueron realizados numerosos esfuerzos para su conservación y/o multiplicación, desarrollándose estudios básicos en aspectos genéticos y fisiológicos. Basado sobre estas lfneas de ciencia básica direccionada a reguladores de crecimiento (fitohormonas), fueron probadas diferentes dosis de ANA (0.00; 0,53 y 1,43 μM) y BAP (2,21; 4,43 y 8,87 JJM) sobre segmentos nodales de cedro provenientes de plántulas in vitro (iniciadas por semilla botánica). Eventos morfológicos de respuesta constatados en altura de planta, número de hojas, número de nudos, número de rafees y longitud de rafees, reportaron que: nulas o pequenas concentraciones de ANA (0,00 • 0,53μM) favorecen el crecimiento longitudinal de rafees y el aumento de número de hojas; asimismo concentraciones de BAP (2,21μM) estimularon preponderantemente et crecimiento de brotes laterales, formación y crecimiento de rafees y el incremento del número de hojas en concentración duplicada (4,43μM). Entretanto, concentraciones de ANA (0,53μM) y BAP (2,21 μM) en función mutua, actúan slnérgicamente sobre el crecimiento y desarrollo de raiz y tallo; siendo que, la ausencia de ANA y duplicación de BAP (4,33μM) actúa de forma positiva sobre el número de nudos y hojas. Existiendo asl, un papel esencial de 6~bencil amino purina (BAP) sobre et crecimiento y desarrollo de segmentos riodales en cedro. Palabras clave: Organogénesis, segmentos nodales, reguladores de crecimiento

Since many decades, forest species coming retaking additive importance. Selective activities in overexploitation practicad during the 80s in the Amazonian regions have to generate great disturbance on population distribution, placing at risk habitats or places of origin. Cedro (Cedrela odorata L.), one of the target species, carne to be considered since 1981 as a priority species for conservation (Lahey, 1995), numerous efforts were made to conservation and/or multiplication, basic research were development on aspects genetics and physiological. Basad about thls efforts and focusing in growth regulators (phytohormones), were tested different doses of ANA (0.00, 0.53 and 1.43 μM) and BAP (2.21, 4.43 and 8.87 μM) on nodal segments from in vitro plants (started by botanical seeds). Events of rnorphological response observed in plant rise, number of leaves, number of nades, number and lengthof roots, reported that: no or low concentrations of ANA (0.00 to 0.53 μM) increase the longitudinal growth of roots and increased number of leaves; also, BAP concentrations (2.21 μM) stimulated predominantly lateral shoot, growth and formation root; the increase in the number of leaves in duplicate concentration (4.43 μM). Moreover, ANA concentrations (0.53 μM) and BAP (2.21 μM) in mutual function, act synergistically on the growth and development of root and stem; being that, the absence of ANA and BAP duplication (4.33 μM) acts positively on the number of nodes and leaves. Exist an essential role of 6-benzylaminopurine (BAP) on the growth and development of segment nodal of cedro.

Country
Peru
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Keywords

580, Propagación, Organogenésis, https://purl.org/pe-repo/ocde/ford#4.01.06

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
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Green