Silk is a special and expensive natural fiber. It enhances added value of products that its used in. Open-end rotor spinning system is the second most favorite spinning system in short staple fiber spinning systems after ring spinning. This spinning system has cost advantages because of its less process needs and high production rate in comparison with ring spinning system. In this study, spinnability of silk fiber in open-end rotor spinning system was investigated. The effects of silk fiber ratio in blends, twist coefficient, rotor speed, rotor type, opening roller type and speed to yarn properties were investigated. Furthermore, single jersey fabrics were produced by Mesdan-lab 294E with medium stitch density and the effect of silk fiber ratio on air permeability, abrasion resistance (through weight loss), surface friction coefficient, thickness and weight of fabrics were investigated. Yarn hairiness and breaking elongation were increased with increasing silk ratio on yarns. Also, air permeability and abrasion resistance of the fabrics were decreased with increasing silk ratio on them.