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Efeito da inoculação via esofágica de microbiota intestinalç sobre a hematologia, desenvolvimento e integridade intestinal de pintos de corte

Authors: Pires, Dayane Lilian;

Efeito da inoculação via esofágica de microbiota intestinalç sobre a hematologia, desenvolvimento e integridade intestinal de pintos de corte

Abstract

Foram avaliados os parâmetros hematológicos de pintos submetidos à inoculação de microbiota intestinal de aves adultas poedeiras e de corte. Para isso, foi feito um experimento para testar o efeito da técnica de inoculação via esofágica. E este não afetou os parâmetros hematológicos. Num segundo experimento, foram utilizados pintos machos recém eclodidos (linhagem COBB). O delineamento experimental foi o inteiramente casualizado, seguindo fatorial 3 x 4, sendo 3 tratamentos [sem inoculação (Controle), inoculação de microbiota intestinal de frangos de corte (IMIFC), inoculação de microbiota intestinal de aves de postura adultas criadas em gaiola (IMIP)] e 4 períodos (1º, 3º, 5º e 7º dia pós-inoculação). A cada período, cinco aves por tratamento foram pesadas e sacrificadas para análise dos seguintes parâmetros: contagem total de eritrócitos (RBC, x106/mm3), hemoglobina (HGB, g/dL), hematócrito (HCT, %), volume corpuscular médio (MCV, μm3), contagem total e diferencial de leucócitos e relação heterófilo:linfócito. Os dados foram submetidos à análise de variância e as médias comparadas pelo Teste de Tukey. Houve resposta temporária dos pintos que receberam inóculo independente do tipo de microbiota. Essa resposta foi observada através do aumento do RBC, HCT e HGB que durou até o 3º dia. Não ocorreu depressão do sistema imune, apenas variação da porcentagem de heterófilos e linfócitos na circulação.

Two experiments were conducted to determine if the technique of esophageal inoculation (Experiment I) and the esophageal inoculation of intestinal microbiota evaluated of adult birds influence the haematological parameters of male broiler chicks. In experiment I, technique of inoculation had no significant effects on haematological parameters [erythrocytes (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), counting total and differential leukocyte and the rate heterophil: lymphocyte]. In experiment II, the experimental design was entirely randomly, following factorial 3 x 4, [3 treatments: no inoculation (Control), inoculation of intestinal microbiota of broiler chickens (IIMBC), inoculation of intestinal microbiota of layer hens (IIMLH); 4 periods: 1, 3, 5 and 7 days post-inoculation]. Inoculation of intestinal microbiota caused a increase in the RBC, HCT and HGB already in the 1 st day post-inoculation. This effect remained until the 3 rd day, but only in the chicks of the treatment IIMBC. In the 3 rd day, an increase in the heterophil percentage and a reduction in the lymphocyte percentage were also observed. The results showed that inoculation of intestinal microbiota caused an immediate and temporary heamatological response.

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Pós-graduação em Zootecnia - FCAV

Country
Brazil
Keywords

Broiler chicks, Intestinal microbiota, Microbiota intestinal, Frango de corte, Hematology, Pintos de corte, Hematologia

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
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Green