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Estudo da microbiota vaginal de éguas com ênfase na pesquisa de lactobacilos

Authors: Chaves, Maria Manoela Barata de Castro;

Estudo da microbiota vaginal de éguas com ênfase na pesquisa de lactobacilos

Abstract

Different from the vagina, were a rich microflora is present, the uterine environment is considered free of microorganisms. One possibility for the installation of endometritis in mares is the ascendent contamination from the vagina.The presence of Lactobacillus on vaginal flora is considered important in woman however there is few information on mares. The present experiment aimed to study the vaginal microflora of embryo donnor mares, to correlate the vaginal and uterine finds and also to determine the prevalence of Lactobacillus on vaginal envirioment. On experiment 1 a total of 77 mares were used and vaginal samples collected from 33 of these mares both vaginal and uterine samples were collected. On experiment 2 vaginal swabs from 36 mares and 10 women were collected for culture and isolation of Lactobacillus, DNA extration and PCR. The predominate bacteria isolated from the vagina were: Streptococcus zooepidemicus (42%), Escherichia coli (25%), Streptococcus alfa hemolítico (15%) Candida (6%), Enterobacter spp (3%), Bacillus spp (3%), Streptococcus beta hemolítico (3%) e Pseudomonas (3%). From 33 samples collected from the uterus only 39% (n= 12) did not show any microorganism on culture. Streptococcus zooepidemicus was the most frequent isolated microorganism (26%), followed by Escherichia coli (15%), Candida spp (9%), Streptococcus alfa hemolítico (6%) e Enterobacter (3%). When evaluated the microorganisms isolated from both vaginal and uterine samples Streptococcus zooepidemicus (21%), Escherichia coli (12%), Candida spp (9%) e Streptococcus alfa hemolytic (6%) were the most frequent isolated bacteria. The agreement between swabs taken from both uterus and vagina was 83.3% (p <0,05) for negative cultures and 73,7% for positive cultures (p <0,05). From 35 samples collected on group I Lactobacillus spp was isolated in only two (5,7%) eight (20%) samples showed positive ... (Complete abstract click electronic access below)

Na égua o ambiente uterino saudável não apresenta microflora, diferente da vagina onde se sabe existir uma flora vaginal rica em microrganismos não patogênicos. Muitas bactérias da flora vaginal normal podem ser deslocadas para o interior do útero, podendo ser esta a causa principal de endometrites em éguas doadoras de embriões. A presença de Lactobacillus spp é considerada importante na flora vaginal de mulheres e tem sido pouco investigada em éguas. O presente trabalho tem como objetivo estudar a flora vaginal de éguas, doadoras de embriões, determinar os principais microrganismos presentes, relacionar os achados microbiológicos vaginais e uterinos, assim como determinar a prevalência de Lactobacillus. No experimento 1 foram utilizadas 77 éguas doadoras de embrião, 33 foram coletadas amostras vaginais e uterinas e 77 apenas vaginais. O experimento 2 contou com dois grupos (36 éguas e 10 mulheres) de swabs vaginais sendo um para cultivo e isolamento de Lactobacillus e outro para extração do DNA e PCR. As bactérias predominantes na vagina foram: Streptococcus zooepidemicus (42%), Escherichia coli (25%), Streptococcus alfa hemolítico (15%) Candida (6%), Enterobacter spp (3%), Bacillus spp (3%), Streptococcus beta hemolítico (3%) e Pseudomonas (3%).. Das 33 amostras coletadas do útero de éguas somente 39% (n=12) não apresentaram crescimento bacteriano ou fúngico. Tendo sido Streptococcus zooepidemicus o mais frequentemente encontrado (26%), seguido de Escherichia coli (15%), Candida spp (9%), Streptococcus alfa hemolítico (6%) e Enterobacter (3%). Os microrganismos isolados da vagina e que estavam concomitantemente presentes no útero de éguas foram: Streptococcus zooepidemicus (21%), Escherichia coli (12%), Candida spp (9%) e Streptococcus alfa hemolítico (6%). Em 83,3% houve concordância entre as amostras negativas na vagina e no útero (p <0,05). Em 73,7%...

Pós-graduação em Medicina Veterinária - FMVZ

Universidade Estadual Paulista (UNESP)

Country
Brazil
Keywords

Lactobacillus, Lactobacilo, Microbiologia veterinaria

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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Green