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Inibidor de tripsina da soja na transferência de imunidade passiva de bezerras da raça holandesa

Authors: Ferreira, Marcela Simões Florio;

Inibidor de tripsina da soja na transferência de imunidade passiva de bezerras da raça holandesa

Abstract

The calves’ immune system is consolidated by the ingestion of colostrum, which is rich in immunoglobulin, the proteins that are responsible for transfering the immunity to the calves. This transferring efficacy is influenced by a series of facts, among them the immunoglobulin’s integrity to be absorbed. Trypsin inhibitors are naturally present on the colostrum and they are able to preserve the immunoglobulin from the proteoclastic enzyme actions. The aim of this work was to evaluate the potentiality of soya Trypsin inhibitor on the passive immunity of the Holstein female calves, by evaluating the concentration of immunoglobulin G (IgG) and total protein (PT) as well as Gammaglutamil transferase (GGT) activity in serum. Eighteen newborn female calves were taken and they were fed with colostrums (T1), colostrums with purified trypsin inhibitor I-S Sigma (50mg), (T2) and colostrums with soya albumin fraction 1.08 g (T3). Blood samples were collected by jugular veined punch within 1, 12, 24, 36 and 48 hours after they were born, the first collection was done before the colostrums was given. Colostrums have presented 3.2% to 7.2 % of fat contents; 14, 10% to 19, 52 % of total protein represented by 7, 84% to 9, 44 % of serum protein (PS). Trypsin inhibitor activities have varied from 0,766 mg to 0,945 mg of inhibited trypsin mL-1. There was no influence of the treatments (p>0,05%) for the analyzed parameters, however, there was (p<0,05%) a significant effect for the period of collection, with an increase on IgG, GGT and, PT concentrations from 0h with the respective medium values of 0,68g.dL- 15,17U.L-1, 4,43 g.dL-1. The absorption pick was at 24 h: with 3,06 g.dL-1, 2.779 U.L-1 and 7,54 g.dL-1 means, respectively for IgG, GGT and PT, indicating an adequate transmission of passive immunity. The addition of soya Trypsin... (Complete abstract click electronic access below)

O sistema imunológico de bezerros consolida-se com a ingestão do colostro, alimento rico em imunoglobulinas que são proteínas responsáveis por transferir imunidade aos bezerros. A eficiência dessa transferência é influenciada por uma série de fatores, dentre eles a integridade das imunoglobulinas a serem absorvidas. No colostro estão naturalmente presentes os inibidores de tripsina, capazes de preservar as imunoglobulinas da ação de enzimas proteolíticas. O objetivo deste trabalho foi avaliar a potencialidade dos inibidores de tripsina da soja na transmissão da imunidade passiva de bezerras da raça Holandesa, por meio da análise das concentrações de imunoglobulina G (IgG) e proteína total (PT) e atividade da gamaglutamiltransferase (GGT) sérica. Foram utilizadas 18 bezerras recém-nascidas alimentadas com colostro (T1), colostro adicionado de inibidor de tripsina purificado Sigma tipo I-S (50 mg) (T2) e provenientes da fração albumina da soja 1,08 g (T3). As amostras de sangue foram colhidas por venopunção da veia jugular com 1, 12, 24, 36 e 48 h após o nascimento, sendo a primeira colheita realizada antes do fornecimento do colostro. O colostro apresentou teor de gordura de 3,2 a 7,2 %, de proteína total de 14,10 a 19,52 %, representada por 7,84 a 9,44 % pelas proteínas do soro (PS). A atividade dos inibidores de tripsina variou de 0,766 a 0,945 mg de tripsina inibida.mL-1. Não houve influencia dos tratamentos (p>0,05%) para os parâmetros analisados, porém houve efeito (p<0,05%) do período de amostragem, com aumento nas concentrações de IgG, GGT e PT a partir da 0h com respectivos valores médios de 0,68 g.dL-1; 15,17U.L-1, 4,43 g.dL-1, com pico de absorção às 24 h: 3,06 g.dL-1, 2.779 U.L-1 e 7,54 g.dL-1, respectivamente, indicando transmissão adequada de imunidade passiva. A adição dos...

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pós-graduação em Zootecnia - FCAV

Country
Brazil
Keywords

Inibidores da tripsina, Imunoglobulinas, Bezerro, Colostrum, Colostro, Albumina

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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