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Efeito da estimulação elétrica e do laser terapêutico na recuperação muscular e nervosa após neurorrafia látero-terminal do nervo fibular em ratos

Authors: Maciel, Fábio Oliveira;

Efeito da estimulação elétrica e do laser terapêutico na recuperação muscular e nervosa após neurorrafia látero-terminal do nervo fibular em ratos

Abstract

INTRODUÇÃO: Os nervos periféricos, assim como os demais tecidos do organismo, estão sujeitos a doenças inflamatórias, traumáticas, metabólicas, tóxicas, genéticas e neoplásicas. Estas doenças levam a diferentes tipos e graus de lesões. As lesões nervosas são bem conhecidas pela redução da capacidade funcional e diminuição da qualidade de vida dos pacientes, essas lesões induzem altos gastos socioeconômicos devido à reabilitação prolongada e absenteísmo dos jovens vítimas de trauma. Questiona-se se a Estimulação Elétrica (EE) e a terapia por Laser de Baixa Potência (LBP) teriam capacidade de melhorar a recuperação muscular e nervosa pós - neurorrafia látero - terminal (NLT). OBJETIVO: Analisar a eficiência da EE e da terapia por LBP na manutenção do músculo tibial cranial (MTC) e regeneração do nervo fibular comum pós NLT. MÉTODO: Trabalho aprovado pelo CEUA FMB - UNESP Nº 1154/2015. Foram utilizados 100 ratos da linhagem Wistar, machos, com massa média de 384,33 g e aproximadamente 9 semanas de vida. Os animais foram divididos em seis grupos experimentais. Grupo Controle, Grupo Desnervado, Grupo com Neurorrafia Látero -Terminal (NLT), Grupo com NLT e Estimulação Elétrica (EE), Grupo com NLT e LASER de baixa potência (LBP) e Grupo com NLT e combinação de EE e LBP (LBP+EE). O tratamento foi realizado durante 180 dias. Foram realizados testes funcionais e histológicos. Análise Estatística: Quando comparados os grupos, foi utilizado predominantemente teste Kruskal Wallis e método de Dunn, ANOVA, seguido pelo teste de TUKEY e o teste de Friedman para avaliar a evolução funcional ao longo do tempo. Em toda a análise, foi utilizado nível de significância p<0,05. RESULTADOS: O Índice Funcional do Fibular (IFF) não mostrou diferença estatisticamente significativa entre os grupos EE, LBP e LBP+EE e o grupo controle. A morfometria muscular mostrou superioridade dos grupos EE e LBP em relação aos grupos NLT e LBP+EE. A morfometria das fibras nervosas mostrou superioridade dos grupos NLT e EE em relação aos grupos LBP e LBP+EE. A razão G mostrou o grupo EE superior aos demais. CONCLUSÃO: Com base em nosso modelo experimental, concluímos que todos os tratamentos puderam promover um gral de recuperação muscular, nervosa e funcional porém o grupo EE se destacou em relação aos demais.

INTRODUCTION: Peripheral nerves, as well as other tissues of the body, are subject to inflammatory, traumatic, metabolic, toxic, genetic and neoplastic diseases. These diseases lead to different types and degrees of injury. Nerve injuries are well known for reducing functional capacity and decreasing patients' quality of life. These injuries induce high socioeconomic costs due to prolonged rehabilitation and absenteeism of young trauma victims.It is questioned whether Electric Stimulation (ES) and Low Power Laser Therapy (LPLT) would have the capacity to improve muscle and nerve recovery after end to side neurorrhaphy (SEN). OBJECTIVE: To analyze the efficiency of ES and LPLT in the maintenance of cranial tibial muscle (CTM) and regeneration of the common fibular nerve post SEN. METHOD: Work approved by certificate No. 1154/2015 CEUA FMB - UNESP. One hundred Wistar rats, male, with a mean mass of 384.33 g and approximately 9 weeks of life, were used. The animals were divided into six experimental groups. Control group, denervated group, group with neurorrhaphy. Grupo Controle, Grupo Desnervado, Group with SEN, Group with SEN and Electrical Stimulation (ES), Group with SEN and low power LASER (LPL) and Group with SEN and combination of ES and LPL (LPL + ES). The treatment was performed for 180 days. Functional and histological tests were performed. Statistical analysis: When comparing the groups, were used predominantly, the Kruskal Wallis test followed by Dunn´s method and ANOVA, followed by the TUKEY test and the Friedman test to evaluate the functional evolution over time. Throughout the analysis, significance level was used p <0.05. RESULTS: The Fibular Functional Index (FFI) showed that there was no statistically significant difference between the EE, LBP and LBP+EE groups and the control group. The muscular morphometry showed superiority of the EE and LBP groups over NLT and LBP+EE groups. The nerve fibers morphometry showed superiority of the NLT and EE groups in relation to the LBP and LBP+EE groups. The G ratio showed the EE group higher than the others. CONCLUSION: Based on our experimental model, we conclude that all treatments could promote a gral of muscular recovery, nervous and functional, but the EE group stood out in relation to the others.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pós-graduação em Bases Gerais da Cirurgia - FMB

Processo FAPESP 2017/19914-3

Country
Brazil
Keywords

Microsurgery, Nervo Fibular Comum, Electrical Stimulation, Regeneração de Nervos, Microcirurgia, Common Fibular Nerve, Nervous Regeneration, Estimulação Elétrica, LASER

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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