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Resistência antimicrobiana em coliformes totais isolados de tanques de refrigeração de leite bovino

Authors: Siqueira, Amanda Keller; Alves, Taila dos Santos; Ferraz, Mirtis Maria Giacini; Franco, Marília Masello Junqueira; Motta, Rodrigo Garcia; Listoni, Fernando José Paganini; Silva, Aristeu Vieira da; +2 Authors

Resistência antimicrobiana em coliformes totais isolados de tanques de refrigeração de leite bovino

Abstract

O leite adequado ao consumo deve possuir qualidade higiênica, valor nutritivo e a manutenção das propriedades organolépticas. O isolamento de coliformes totais e/ou fecais de leite bovino é considerado um indicador de sanidade e de boas práticas de manejo, podendo ser usado como indicador de qualidade. Este estudo teve como objetivo isolar, identificar e observar o perfil de resistência de coliformes presentes em tanques de refrigeração coletivos e individuais de acondicionamento de leite. Foram colhidas 89 amostras de leite de tanques coletivos e isolados 21 Klebsiella spp., uma Escherichia coli (E. coli) e 29 Enterobacter spp., e 102 amostras de leite de tanques individuais das quais foram isolados uma Klebsiella spp. e sete Enterobacter spp. Dos tanques coletivos, aproximadamente 47% de Klebsiella spp. e Enterobacter spp. foram resistentes para cefalexina e 30% para ampicilina. Destes, no mínimo 24% apresentaram multirresistência. Entre os isolados de tanques individuais, 85% ou mais apresentaram resistência para ampicilina. O fenótipo ESBL e o gene blaTEM foram detectados em linhagens de Klebsiella spp. isoladas de ambos os tanques. Constatou-se contaminação do leite com coliformes totais resistentes e, principalmente, que o armazenamento do leite cru de vários pequenos produtores em tanques coletivos potencializa o risco de contaminação.

Milk that is adequate for consumption must be of hygienic quality, nutritional value, and should maintain its organoleptic properties. Isolation of fecal and/or total coliforms from bovine milk is considered an indicator of hygiene and good management practices, and can be used as a quality indicator. This study aimed to isolate, identify, and assess the resistance profile of coliforms isolated from collective bulk tanks and individual milk tanks. A total of 89 milk samples were collected from collective bulk tanks and, from these, 21 Klebsiella spp., one E. coli, and 29 Enterobacter spp. were isolated, whereas 102 milk samples from individual tanks showed isolation of one Klebsiella spp. and seven Enterobacter spp. In collective bulk tanks, at least 47% of Klebsiella spp. and Enterobacter spp. were resistant to cephalexin and 30% to ampicillin. From these, at least 24% showed multidrug resistance. Among the microorganisms isolated from the individual tanks, 85% or more were resistant to ampicillin. The ESBL phenotype and the blaTEM gene were detected in strains of Klebsiella spp. isolated from both tanks. It was concluded that contamination of milk with resistant total coliforms, and especially the storage of raw milk from several small producers in the collective bulk tank increase the risk of contamination.

Country
Brazil
Keywords

Multirresistência, Klebsiella spp, Klebsiella spp., Enterobacter spp., Vacas de leite, Enterobacter spp, E. coli, Dairy cow, Multiresistance

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
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Green