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Biosíntesis de Glutamato en "Chlamydomonas reinhardii" purificación y propiedades de las encimas con actividad Glutamato Sintasa

Authors: Márquez Cabeza, Antonio José;

Biosíntesis de Glutamato en "Chlamydomonas reinhardii" purificación y propiedades de las encimas con actividad Glutamato Sintasa

Abstract

De forma resumida, los puntos más importantes que aporta este trabajo son los siguientes: 1. Puesta a punto t caracterización de un ensayo adecuado para medir la actividad GOGAT en Chlamydomonas reinhardii, tanto in situ como in vitro. 2. Estudios in vivo de asimilación de amonio, utilizando distintas estirpes de Chlamydomonas reinhardii, y efecto de distintas condiciones metabólicas sobre el nivel de las actividades NADH- y Fd-GOGAT. 3. Purificación parcial de la NADH-GOGAT y estudio físico-químico y cinético de la proteína. 4. Purificación hasta homogeneidad, caracterización fisicoquímica y cinética, y estudio de la composición de grupos prostéticos de las proteínas del sistema ferredoxina/Fd-GOGAT.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green