N-Me-pAB-Glu-gamma-Glu-gamma-Tyr(3-NO(2)): an internally quenched fluorogenic gamma-glutamyl hydrolase substrate.
Copyright policy )N-Me-pAB-Glu-gamma-Glu-gamma-Tyr(3-NO(2)): an internally quenched fluorogenic gamma-glutamyl hydrolase substrate.
A gamma-glutamyl tripeptide containing an internally quenched fluorophore has been synthesized and shown to be a substrate for recombinant rat gamma-glutamyl hydrolase. HPLC, LC-MS, and fluorescence spectra support the conclusion that selective hydrolysis occurs at the penultimate peptide bond. Preliminary data indicate that hydrolysis of this substrate can be monitored continuously to yield steady-state kinetic data.
- University of Michigan–Flint United States
Hydrolysis, gamma-Glutamyl Hydrolase, Mass Spectrometry, Recombinant Proteins, Rats, Kinetics, Structure-Activity Relationship, Spectrometry, Fluorescence, Animals, Oligopeptides, Chromatography, High Pressure Liquid, Fluorescent Dyes
Hydrolysis, gamma-Glutamyl Hydrolase, Mass Spectrometry, Recombinant Proteins, Rats, Kinetics, Structure-Activity Relationship, Spectrometry, Fluorescence, Animals, Oligopeptides, Chromatography, High Pressure Liquid, Fluorescent Dyes
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).5 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!