
The synthesis of UDP-glucose-6-s-H was performed through condensation of alpha-D-glucopyranosyl phosphate-6-3-H and uridine 5'-phosphomorpholidate. Enzymic oxidation of UDP-glucose-6-3-H with calf liver UDP-glucose dehydrogenase was found to proceed with direct transfer of the hydrogen from C-6 of UDP-glucose onto NAD.
Uridine Diphosphate Glucose, Chromatography, Paper, Chromatography, Ion Exchange, Tritium, Uridine Diphosphate Glucose Dehydrogenase, Uridine Diphosphate Sugars, Catalysis, Alcohol Oxidoreductases, Liver, Spectrophotometry, Animals, Scintillation Counting, Cattle, Oxidation-Reduction
Uridine Diphosphate Glucose, Chromatography, Paper, Chromatography, Ion Exchange, Tritium, Uridine Diphosphate Glucose Dehydrogenase, Uridine Diphosphate Sugars, Catalysis, Alcohol Oxidoreductases, Liver, Spectrophotometry, Animals, Scintillation Counting, Cattle, Oxidation-Reduction
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