The assessment of vascular stiffness is gaining significance in cardiovascular research due to the need to analyze the properties of the arterial system and perform clinical evaluations of patients. Ongoing research seeks to resolve technical and methodological challenges in measuring arterial stiffness, aiming to enhance its reliability and clinical relevance. This second edition highlights recent advancements in the methodologies employed to assess vascular stiffness, along with both preclinical and clinical applications. It features new data and in-depth reviews on the use of arterial stiffness and early vascular aging measures, particularly in addressing issues such as risk stratification in special populations and specific diseases. Recent technical innovations in the field also occupy a special place in this reprint, making it a vehicle for disseminating important advancements in the field of vascular stiffness.