The authors report the distribution of Nymphaea alba L. in Tuscany by the analysis of herbarium specimens, bibliographic references and records on field. The study shows two different situations: the species is distributed almost exclusively along the wetland's net on the northwestern floodplains in the district of Florence, Pistoia, Lucca, Pisa and Massa. Except for few and isolated sites, in the districts of Arezzo, Siena and Grosseto, the species seems not to occur in the rest of Tuscan wetlands. © 2018 Società Botanica Italiana onlus.