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Article . 2017
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Evaluación de la transferencia pasiva de la inmunidad en equinos mediante el uso de diferentes pruebas

Authors: Carabetta, Daiana; Fernández, Daniel; Etcheverría, Analía Inés; Valle, Marcelo; Padola, Nora Lía;

Evaluación de la transferencia pasiva de la inmunidad en equinos mediante el uso de diferentes pruebas

Abstract

El objetivo de este trabajo fue utilizar diferentes métodos de evaluación de la inmunidad tanto en calostro de yeguas como en suero de sus crías, analizar beneficios y desventajas de cada uno y determinar cuál de éstos es el más adecuado para el uso en temporada de partos. Se tomaron 13 muestras de calostro y suero y se realizaron las siguientes pruebas: evaluación subjetiva del calostro por su aspecto, evaluación de la densidad específica mediante calostrómetro, concentración de azúcares y proteínas totales por refractometría y determinación de la concentración de IgG con glutaraldehído (Inmuno-G Test®). Se observó que dos potrillos tuvieron bajas concentraciones de IgG, debido probablemente a que ninguno calostró bien a causa de pérdida de calostro por la madre antes del parto. Determinamos que la prueba de precipitación con glutaraldehído es la más conveniente a incluir, por ser precisa, rápida y fácil de realizar, sobre todo en establecimientos con muchos animales, evitando gastos en laboratorio para la determinación de gammaglobulinas. Esto los hace más económicos. Las pruebas restantes no serían obsoletas pero no se aconseja realizarlas como pruebas individuales para determinar calidad de calostro o una buena transferencia de inmunidad. Sí se pueden realizar como pruebas complementarias.

The aim of this study was to evaluate different methods of detecting IgG both mares colostrum and serum of their pups, to analyse benefits and drawbacks of each against each other and determine which of them is best suited for use in season delivery. Thirteen colostrum and serum samples were taken to realize the following tests: Subjective evaluation of colostrum in appearance, evaluation of the specific density by colostrometer, sugar concentration and total protein by refractometry and determination of the concentration of IgG with glutaraldehyde. The data obtained was observed that two foals had low concentrations of IgG, probably because none colostrum either because of loss of colostrum by the mother before delivery. We determined that the precipitation test with glutaraldehyde is the most convenient to include, to be precise, quick and easy to perform, especially in places with many animals, avoiding expenses laboratory gamma globulins. This makes them cheaper. The remaining tests would not be advisable obsolete but not perform them as individual testing to determine quality of colostrum or a good transfer of immunity, you can make as complementary tests.

Fil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Fernández, Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina

Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina

Fil: Carabetta, Daiana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina

Fil: Valle, Marcelo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina

Country
Argentina
Keywords

SUERO, https://purl.org/becyt/ford/4.3, https://purl.org/becyt/ford/4, CALOSTRO, COAGULACIÓN CON GLUTARALDEHÍDO

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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