Geoffroea decorticans, popularly known as "chañar", is traditionally used as anti-inflammatory, to treat infected wounds and dysentery. Chañar bark is an external tissue known for its medicinal properties, but its bioactive components were not reported yet. Objectives: To evaluate the antibacterial activity of polyphenolics (PG) and triterpenoids (TG) enriched fractions obtained from Geoffroea decorticans bark (CC) against Escherichia coli and Staphylococcus aureus. Methodology: CC grounded powder was washed with n-hexane and extracted with methanol. The extract was dried and fractionated by precipitation with acetone to separate TG from PG. The extracted material (EM) was determined for TG and PG. Antibacterial activity was evaluated against E. Coli (ATCC 25922) and S. aureus (ATCC 29213) by dot-blot bioautography and disk diffusion assay. Samples were assayed at 250 - 750 μg of EM, Ciprofloxacin was used as control. The plates were incubated at 37° C for 24 h. The bioactive fractions were analyzed by GC-MS. Results: PG (250 μg) was active against both strains. The inhibitory halo obtained against S. aureus was significant higher than for E. coli (p>0.05). TG did not showed activity against the assayed strains. GC-MS experiments performed for PG showed the presence of Dihydroquercetin, 3,4´-Dimethoxy-5,7,3´-trihydroxyflavone and protocatechic acid. Conclusions: CC exhibited antibacterial activity against Gram positive and Gram negative strains, which support ethnomedicinal use. This activity could be attributed to the phenolic compounds constituents.

Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina

Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina

Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina

Fil: Iriarte, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina

XXXVI Annual Scientific Meeting of the Tucuman Biology Association

Asociación de Biología de Tucumán

Tafí Viejo

Argentina