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Article . 2023
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Abordaje quirúrgico al testículo en el armadillo Chaetophractus villosus (Dasypodidae, Xenarthra)

Authors: Iodice, Omar Hector; Luaces, Juan Pablo; Cervino, Claudio Osvaldo;

Abordaje quirúrgico al testículo en el armadillo Chaetophractus villosus (Dasypodidae, Xenarthra)

Abstract

Armadillos have been used in biomedical research across various disciplines, classified as Non-Traditional Laboratory Animals (NTLAs). They are eutherian mammals with intraabdominal testes (IAT). This study describes a surgical technique to access IAT in the Chaetophractus villosus armadillo (“large hairy armadillo”). Fifteen adult specimens were used, maintained in a vivarium at a room temperature of 22 ± 2°C, under a 12:12 light-dark photoperiod, and fed dry canine food. Anesthesia was induced using ketamine + xylazine + diazepam + atropine (60 mg/kg, 3 mg/kg, 1 mg/kg, 0.05 mg/kg, respectively) administered intramuscularly, achieving appropriate surgical anesthesia. The depth of anesthesia was assessed through righting and withdrawal reflexes. The surgical technique was divided into three stages: pre-surgical, surgical, and post-surgical, with the surgical stage further subdivided into four phases (dissection, exposure, puncture or excision, and synthesis). The anesthetic induction time was 6 ± 1 minutes, surgical anesthesia lasted 38 ± 7 minutes, incision and puncture took 12 ± 2 minutes, and orchiectomy took 19 ± 5 minutes. Anesthetic recovery was achieved in 5 ± 1 hours, and surgical wound healing occurred in 6 ± 1 days, allowing for the removal of external sutures in 9 ± 1 days. This experimental technique proved successful for its intended purposes. It closely resembles the approach used in orchiectomies for animals with cryptorchidism. It is considered suitable for performing other types of laparotomies in armadillos and may be valuable for future studies related to IAT

Los armadillos han sido utilizados en la investigación biomédica en distintas disciplinas siendo considerados Animales de Laboratorio No Tradicionales (ALNT). Son mamíferos euterios con testículos intraabdominales (TIA). Este estudio describe una técnica quirúrgica para acceder a TIA en el armadillo Chaetophractus villosus (“peludo”). Se emplearon 15 ejemplares adultos mantenidos en bioterio con temperatura ambiente 22 ± 2ºC, fotoperíodo L:O 12:12 y alimentación seca para caninos. Se los anestesió con ketamina + xilacina + diazepam + atropina (60 mg/kg, 3 mg/kg, 1 mg/kg, 0.05 mg/kg respectivamente) por vía intramuscular, logrando una anestesia quirúrgica adecuada. La profundidad de la anestesia se evaluó mediante reflejos de enderezamiento y retirada. La técnica quirúrgica se dividió en tres etapas: prequirúrgica, quirúrgica y postquirúrgica, con la etapa quirúrgica subdividida en cuatro tiempos (diéresis, exposición, punción o exéresis y síntesis). El tiempo de inducción anestésica fue de 6 ± 1 minutos, la anestesia quirúrgica duró 38 ± 7 minutos, la incisopunción llevó 12 ± 2 minutos y la orquiectomía 19 ± 5 minutos. La recuperación anestésica se logró en 5 ± 1 horas, y la cicatrización de la herida quirúrgica ocurrió en 6 ± 1 días, permitiendo la retirada de los puntos externos en 9 ± 1 días. Esta técnica experimental resultó exitosa a los fines propuestos. Es similar a la utilizada en orquiectomías en animales con criptorquidia. Se considera adecuada para realizar otros tipos de laparotomías en armadillos y puede ser valiosa para futuros estudios relacionados con el TIA.

Fil: Luaces, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina

Fil: Iodice, Omar Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Moron. Secretaria de Ciencia y Tecnologia. Instituto de Fisiologia y Neurociencias.; Argentina

Fil: Cervino, Claudio Osvaldo. Universidad de Moron. Secretaria de Ciencia y Tecnologia. Instituto de Fisiologia y Neurociencias.; Argentina

Country
Argentina
Keywords

ANIMALES DE LABORATORIO NO TRADICIONALES (ALNT), CIRUGÍA TESTICULAR, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/4.3, CHAETOPHRACTUS VILLOSUS, TESTÍCULO INTRABDOMINAL, ANESTESIA, https://purl.org/becyt/ford/4, https://purl.org/becyt/ford/1

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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