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Estudios citogenéticos en el complejo poliploide Zephyranthes mesochloa Herb. (Amaryllidaceae)

Authors: Gianini Aquino, Analía Cecilia; Rodriguez Mata, Orlando Abrahan; Honfi, Ana Isabel; Daviña, Julio Rubén;

Estudios citogenéticos en el complejo poliploide Zephyranthes mesochloa Herb. (Amaryllidaceae)

Abstract

Background and aims: Zephyranthes mesochloa, is a bulb species with white flowers that inhabits the subtropical regions of South America and presents different chromosome numbers. Different chromosome numbers have been reported for this species. The aim of this work is to perform chromosome analysis of specimens of Z. mesochloa from northern Argentina, in order to contribute to cytogenetic knowledge of the genus.M & M: Ploidy levels and somatic chromosome numbers were determined in individuals belonging to 8 populations from northern Argentina using conventional staining. In addition, meiotic preparations with acetic carmine in one of the individuals, were made.Results: Seventy five percent of the populations were diploid, with 2n = 2x = 12 with a karyotypic formula 4m + 4sm + 4st. In two of the diploid populations, individuals with 2n = 13 were observed. These individuals presented an additional metacentric chromosome, a B chromosome. Analysis of diakinesis and metaphase I of aneuploid plants microsporogenesis (2n = 13) showed 6 bivalents and 1 univalent in all pollen mother cells (PMCs) analyzed. The remaining populations (25%) were tetraploids with 2n = 4x = 24 and a karyotypic formula 8m + 8sm + 8st. Geographically, the distribution of Z. mesochloa cytotypes are discontinuous, without sympatric or adjacent combinations.Conclusions: The duplicate karyotype formula in tetraploids regarding its con-specific diploids and the high morphological identity between the 2x and 4x accessions, indicate that the origin of an autopolyploid cytotype. The available data allows us to consider the group of cytotypes as part of a polyploid complex with a basic number of x = 6.

Introducción y objetivos: Zephyranthes mesochloa es una especie bulbosa de flores blancas que habita en la región subtropical del sur de Sudamérica y registra distintos números cromosómicos. El objetivo del presente trabajo es analizar cromosómicamente individuos de la especie Z. mesochloa del norte de Argentina, con el objeto de contribuir al conocimiento citogenético del género. M&M: El nivel de ploidía y el número cromosómico somático se determinó en individuos procedentes de 8 poblaciones del norte de Argentina a partir de tinción convencional. Además, se realizaron preparaciones meióticas con carmín acético en individuos de una de las poblaciones. Resultados: El 75% de las poblaciones resultaron diploides, con 2n = 2x = 12 y fórmula cariotípica 4m + 4sm + 4st. En dos de las poblaciones diploides se observaron individuos con 2n = 13, con presencia de un cromosoma metacéntrico adicional, que posiblemente se trate de un cromosoma B. El análisis de la microsporogénesis, en diacinesis y metafase I, del citotipo aneuploide (2n = 13), mostró 6 bivalentes y 1 univalente en todas las células madre del polen analizadas. Las poblaciones restantes (25%), resultaron tetraploides con 2n = 4x = 24 y fórmula cariotípica 8m + 8sm + 8st. Geográficamente, la distribución de los citotipos de Z. mesochloa es discontinua. Conclusiones: La fórmula cariotípica duplicada en los tetraploides con respecto a los diploides conespecíficos indicarían un origen autopoliploide de este citotipo. El conjunto de datos disponibles permite considerar al conjunto de citotipos como un complejo poliploide con número básico x = 6.

Fil: Rodriguez Mata, Orlando Abrahan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina

Fil: Gianini Aquino, Analía Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina

Fil: Daviña, Julio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina

Fil: Honfi, Ana Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina

Country
Argentina
Keywords

karyotype, chromosomes, ornamental bulb plant, https://purl.org/becyt/ford/1.6, autopolyploidy, meiosis, B chromosome., https://purl.org/becyt/ford/1

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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