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Estudios citogenéticos en especies de Passiflora subgénero Passiflora (Passifloraceae)

Authors: Chiapero, Ana Laura; Las Peñas, Maria Laura; Amela Garcia, Maria Teresa; Bernardello, Gabriel Luis Mario;

Estudios citogenéticos en especies de Passiflora subgénero Passiflora (Passifloraceae)

Abstract

Passiflora comprende alrededor de 525 especies con x = 9 ó 6. Se realizó un estudio citogenético de cuatro especies del subgénero Passiflora que crecen en la Argentina. Se utilizaron las técnicas de Giemsa, bandeo cromosómico fluorescente CMA/DAPI y FISH (sonda 18-5,8-26S y 5S) en cromosomas mitóticos. P. caerulea, P. tucumanensis y P. cincinnata resultaron diploides (2n = 18) mientras que P. mooreana, tetraploide (2n=36). Los cariotipos fueron altamente simétricos con la fórmula 8 m + 1 sm, duplicada en P. mooreana. Con CMA/DAPI, todos los diploides presentaron dos o tres pares de bandas terminales CMA+ /DAPI- , mientras que la especie tetraploide presentó cinco pares, asociadas a NOR. Con FISH, los loci 18-5,8-26S coincidieron en número y posición con las bandas CMA+ /DAPI- . En cada especie, el número de sitios para los genes de 5S ADNr concordaron con el nivel de ploidía. Los datos de P. tucumanensis y P. mooreana se reportan por primera vez, siendo esta última el primer tetraploide conocido para el subgénero Passiflora. Los patrones de FISH y las bandas heterocrómaticas variaron entre las especies de Passiflora estudiadas, principalmente en el número y distribución de las señales.

Cytogenetic studies in species of Passiflora subgenus Passiflora (Passifloraceae). Passiflora has nearly 525 spp. with x = 9 or 6. A cytogenetic study of four species from subgenus Passiflora thatgrow in Argentina was performed. Giemsa technique, fluorescent chromosome banding CMA/DAPI and FISH (probe 18-5,8-26S and 5S) were used in mitotic chromosomes. P. caerulea, P. tucumanensis and P. cincinnata resulted diploid (2n = 18) while P. mooreana tetraploid (2n = 36). The karyotypes were highly symmetrical, with the formula 8 m + 1 sm, duplicated in P. mooreana. With CMA/DAPI, diploids showed 2-3 pairs of terminal bands CMA+DAPI', whereas the tetraploid five bands, associated to NOR in all cases. With FISH, the loci 18-5.8-26S coincided in number and position with the CMA+/DAPI- bands. In each species, the number of sites for 5S rDNA genes agreed with the ploidy level. Data of P. tucumanensis and P. mooreana are here reported for the first time, being this last species the first known tetraploid for subgenus Passiflora. FISH patterns and heterochromatic bands varied among the Passiflora species studied, mainly with respect to the number and distribution of the signals.

Fil: Amela Garcia, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Plantas Tóxicas y Medicinales, Metabolismo de Compuestos Sintéticos y Naturales - Hongos que Intervienen en la Degradación Biológica; Argentina

Fil: Chiapero, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina

Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina

Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina

Keywords

Evolución, Heterocromatina, Fish, Passiflora, Adnr 5s And 18-5,8-26s, https://purl.org/becyt/ford/1.6, Citogenetica, Cariotipos, https://purl.org/becyt/ford/1

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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