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Apomixis expression in Eragrostis curvula under stress conditions

Authors: Zappacosta, Diego Carlos; Ochogavía, Ana Claudia; Romero, José Rodolfo; Rodrigo, Juan Manuel; Meier, Mauro Sebastián; Polci, Pablo Aldo; Garbus, Ingrid; +2 Authors

Apomixis expression in Eragrostis curvula under stress conditions

Abstract

Over the past few years our research group has been analyzing the molecular mechanisms involved in the reproductive control of weeping lovegrass (Eragrostis curvula [Schrad.] Nees), an apomictic perennial grass. This work was aimed at analyzing apomixis expressivity in Eragrostis curvula plants subjected to three stress conditions: 1) tissue culture followed by a colchicine-induced ploidy change; 2) tissue culture and 3) water deficit. In the first system, a colchicine-induced polyploid plant (C) changed the expressivity of apomixis in a four year period, being highly sexual shortly after polyploidization and highly apomictic four years later. During this interval, genetic and epigenetic changes were also observed. While genetic variation led to diversification with respect to an obligate apomictic control, epigenetic variation resulted in increased similarity. Polymorphic sequences were mainly similar to transposable elements and/or protein-coding-like sequences. In the second system, explants originated from near obligate apomictic plants were subjected to tissue culture. The expressivity of apomixis of a regenerated plant was analyzed immediately after treatment and nine years later. In this period, the levels of sexuality shifted from 45 to 0%. In the third model, near-obligate apomictic plants subjected to water stress showed an increase in expression of sexuality from 2 to 20%. As a whole, our results suggested that stress can temporarily activate sexuality in apomictic genotypes. At least in one system, this change was correlated with genetic and epigenetic modifications involving retroelements and/or protein-coding-like sequences.

Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Country
Argentina
Keywords

APOMIXIS, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1, STRESS CONDITIONS, ERAGROSTIS CURVULA, EPIGENETICS

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green