Apomixis, defined as asexual propagation by seeds, is a type of plant reproduction found in more than 400angiosperms. This polyphyletic trait has been studied in various species, and it is considered of greatimportance in the agricultural industry as it would allow the fixation of desired traits and its propagationthrough generations. The study of ovule development in an apomictic model is necessary to strengthen theknowledge of the mechanisms governing this reproductive mode, and would contribute to improve plantbreeding procedures. Weeping lovegrass (Eragrostis curvula (Schrad.) Ness) is a perennial grass that has beenextensively studied as a model species for diplosporous apomixis. This species, mostly used as forage insemi-arid regions of the world, comprises a polymorphic complex that includes sexual and apomicticcytotypes, where all apomicts are polyploids, ranging from tetraploids (4X) to octoploids (8X). The aim of thiswork was to provide a thorough description of the developmental stages taking place in the ovule of threetetraploid genotypes of weeping lovegrass: the full apomictic Tanganyika, the facultative apomictic DonWalter, and the sexual OTA, as well as evaluating pollen development, using confocal laser microscopy.Moreover, in order to further understand the mode of reproduction in this species, anin-situ hybridization (ISH)was performed using an SPL gene (Squamosa Promoter-binding-Like), found to be differentially expressedbetween two of the contrasting genotypes (OTA-S and Tanganyika). The present analysis of the femalegametophyte aided in increasing the knowledge of the reproductive development in E. curvula and allowed theidentification of differences between sexual and apomictic development.

Fil: Diaz, Alejandra Raquel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina

Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

Fil: Mendes, Marta Adelina. Università degli Studi di Milano; Italia

Fil: Mizzotti, Chiara. Università degli Studi di Milano; Italia

Fil: Cucinotta, Mara. Università degli Studi di Milano; Italia

Fil: Colombo, Lucia. Università degli Studi di Milano; Italia

Instituto de Investigaciones en Ciencias Agrarias de Rosario