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Article . 2012
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Determinación de los puntos de corte para el diagnóstico de endometritis subclínica en Argentina

Authors: Madoz, Laura Vanina; Plöntzke, J.; Albarracín, D.; Drillich, M.; de la Sota, Rodolfo Luzbel;

Determinación de los puntos de corte para el diagnóstico de endometritis subclínica en Argentina

Abstract

La endometritis subclínica (ES) afecta negativamente a la reproducción de las vacas de tambo al aumentar el intervalo entre partos. Para su diagnóstico, se debe tomar una muestra citológica del útero y determinar el porcentaje de neutrófilos (PPMN) que se encuentra superficialmente en el endometrio. Distintos PPMN han sido utilizados como puntos de corte de diagnóstico en vacas estabuladas. No es posible extrapolar datos de explotaciones intensivas a las condiciones argentinas de producción, tampoco el uso de valores de corte arbitrarios, debido a que se pueden llegar a interpretar en forma errónea los resultados arribando a diagnósticos incorrectos. Por lo tanto, el objetivo de este trabajo fue determinar la prevalencia de la ES diagnosticada por la técnica de CB en Argentina y buscar los niveles de corte que se correspondan con una disminución de la eficiencia reproductiva. Se muestrearon y evaluaron 487 vacas con flujo vaginal normal entre 21 y 62 días postparto (dpp). Los datos fueron analizados por SAS y SigmaPlot. Las curvas ROC (acrónimo de Característica Operativa del Receptor) obtenidas arrojaron valores de corte de 8, 6, 4 y 5 PPMN para los intervalos 21-33, 34-47, 48-62 y 21-62 dpp respectivamente. Las vacas con ES tuvieron menor tasa de concepción al primer servicio (16,2%) y tuvieron 29 días más de abiertas comparados con vacas sin ES.

Subclinical endometritis (SE) adversely affects the reproduction of dairy cows by increasing the number of days open. For diagnosis of subclinical endometritis, it is necessary to take a cytological sample of the uterus and determine the percent of neutrophils (PPMN) present at the endometrial surface. Different percentages have been used as diagnostic cutoffs in dairy cows. It is not possible to extrapolate data of intensive productions to Argentine pastoral conditions, or to use arbitrary cutoff values. This could result in erroneous interpretation of results and misdiagnosis of subclinical endometritis. The aim of this study was to validate cytobrush technique in our country and find cutoff values which correspond to a real decline in reproductive efficiency. For this work 487 cows with normal vaginal discharge between 21 and 62 days postpartum (dpp) were sampled and subclinical endometritis was diagnosed. Data were analyzed by SAS and Sigma Plot. ROC (Receiver Operating Characteristic) curves generated cutoff values of 8, 6, 4 and 5 PPMN for the intervals 21-33, 34-47, 48-62 and 21-62 dpp, respectively. Cows with subclinical endometritis had a 16.2% reduction in first conception rate and had 29 more open days than cows without subclinical endometritis.

Fil: de la Sota, Rodolfo Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina

Fil: Madoz, Laura Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina

Fil: Albarracín, D.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina

Fil: Plöntzke, J.. Freie Universitat Berlin (freie Universitat Berlin); Alemania

Fil: Drillich, M.. Universidad de Medicina Veterinaria; Austria

Country
Argentina
Keywords

Vacas lecheras, Endometritis subclinica, https://purl.org/becyt/ford/4.3, https://purl.org/becyt/ford/4, Puntos de corte

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green