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International Journal of Environment Agriculture and Biotechnology
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Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2021 Argentina Publisher:AI PublicationsJournal:International Journal of Environment, Agriculture and Biotechnology, volume 6, pages 196-205 (eissn: 2456-1878, Copyright policy )
Authors: Silvina Soledad Maidana; Maria Mercedes Odeon; Carola Maria Ferrecio; Noelia Magali Grazziotto; Eddie Pisano; Irene Alvarez; Lucia Rocha; +2 Authors

doi: 10.22161/ijeab.66.24

handle: 11336/185019

Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

Abstract

Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide an usefultool for screening animals for the presence of antibodies (Abs) against a wide range of infectious agents (including viruses that cause respiratory disease in cattle) and are mainly used in veterinary medicine to assist to the control and disease?s monitoring. The aim of the present study was developing and validating one indirect enzyme-linked immunosorbent assay (ELISA) based on semi purified bovine parainfluenza virus type 3 (BPIV3).This test would allow to detect and quantify Abs against PI3 in serum sample from cattle and guinea pigs on both purposes diagnostic and typify/specify the quality of vaccines. The diagnostic sensitivity and specificity from the assay was 88% and 100% for bovine samples, using a threshold of corrected optical density, ODc =0.300, and 91% and 100% for guinea pig samples with a ODc =0.250.The intermediate precision expressed as the assays positive control coefficient of variation (CV) was 20% for bovines and 8.5% for guinea pigs. Both techniques reproducibility obtained in interlaboratory assays was CV=17% for bovines and 15% for guinea pigs, which found the requirements of OIE (CV<30%). The efficacy of biological medicinal products, such as vaccines, relies an optimal model testing quality control. The validated ELISAs represents an important tool for testing vaccine quality, and quantifying and controling BPIV3 infections on cattle.

Fil: Romera, Sonia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Universidad del Salvador; Argentina

Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina

Fil: Odeon, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina

Fil: Ferreccio, Carola Maria. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina

Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina

Fil: Alvarez, Irene. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina

Fil: Grazziotto, Noelia Magalí. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina

Fil: Rocha, Lucia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina

Country
Argentina
Related Organizations
Keywords

PI3, https://purl.org/becyt/ford/4.3, GUINEA PIG, BOVINE, ELISA, https://purl.org/becyt/ford/4

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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