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CONICET Digital
Article . 2018
License: CC BY NC
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Complejos vitamina E-β-lactoglobulina incluidos en películas biopoliméricas de alginato de sodio

Authors: Berino, Romina Paola; Pezzelatto, Diego Armando Francisco; Báez, Germán David; Llopart, Emilce Elina; Ballerini, Griselda A.; Moro, Andrea; Delorenzi, Nestor Jorge; +1 Authors

Complejos vitamina E-β-lactoglobulina incluidos en películas biopoliméricas de alginato de sodio

Abstract

Se optimizaron las condiciones para la preparación de películas biopoliméricas capaces de encapsular los valores diarios máximos recomendados de la vitamina liposoluble E (α-tc), incorporando nanocomplejos formados por la proteína láctea beta lactoglobulina (β-lg) y la vitamina en soluciones alginato de sodio (AS), a una relación β-lg/AS determinada previamente. En primer término, se estudió por turbidimetría la interacción entre β-lg (nativa y desnaturalizada por calor) con α-tc, a diferentes pHs, y se comprobó la eficacia de β-lg nativa a pH 6,80 para solubilizar la α-tc en soluciones acuosas. Se realizaron ensayos de opacidad y color de las películas y no se encontraron diferencias por la incorporación del α-tc. Se evaluó por HPLC la integridad de la estructura de α-tc en los nanocomplejos formados en soluciones acuosas y en las películas de alginato a lo largo de un período de tiempo. La película seca mejoró la protección de la estructura del α-tc al ser expuesta al oxígeno del aire y luz controlada, efecto atribuible a la barrera al oxígeno y luz ultravioleta (UV) que ofrecen las películas secas de polisacáridos.

In order to obtain dried films capable of encapsulating the maximum recommended daily values of fat-soluble vitamin E (α-tc), nanocomplexes between beta-lactoglobulin (β-lg), the major whey protein, and the vitamin were incorporated into sodium alginate (AS) solutions, to previously determined β-lg / AS ratio. The interaction between native and heat-treated protein with vitamin was studied by turbidimetry at different pH values. Native β-lg increased solubility α-tc in aqueous solutions at pH 6.80. Opacity and color tests to all films were carried out and no differences were found due to the incorporation of α-tc. Dry films improved the protection of α-tc structure when exposed to oxygen from the air and controlled light (measured by HPLC). This effect was attributable to the oxygen and ultraviolet light (UV) barrier exerted by the polysaccharide matrix.

Fil: Ballerini, Griselda A.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de los Alimentos; Argentina

Fil: Berino, Romina Paola. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina

Fil: Báez, Germán David. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina

Fil: Llopart, Emilce Elina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina

Fil: Pezzelatto, Diego Armando Francisco. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina

Fil: Delorenzi, Nestor Jorge. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina

Fil: Busti, Pablo Andres. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina

Fil: Moro, Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología; Argentina

Country
Argentina
Keywords

NANOCOMPLEJO, https://purl.org/becyt/ford/2.11, PELICULAS, ALGINATO DE SODIO, https://purl.org/becyt/ford/2, BETA-LACTOGLOBULINA

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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