
handle: 11336/147099
O objetivo do presente trabalho foi avaliar o efeito do protocolo J-Synch na taxa de prenhez (TP) de novilhas artificialmente inseminadas após a ocorrência de estro (DEAI) ou em tempo fixo (FTAI). Duzentas e noventa e três novilhas Braford foram usadas. No dia 0, todos os animais receberam 2,0 mg de benzoato de estradiol administrado por via intramuscular (IM) e um dispositivo intravaginal de liberação de progesterona (DIB, 0,5 g). As novilhas foram distribuídas nos seguintes grupos: 1) J-Synch: no dia 6, os dispositivos DIB foram removidos e 500 µg de d-Cloprostenol sódico (d-CS) foi administrado IM e a tinta foi aplicada na base da cauda (tinta da cauda). No dia 9 (60 h após a remoção do DIB), as novilhas com a tinta removida (≥ 40%) foram inseminadas. Aquelas que não apresentaram estro em 72 horas após a remoção do DIB receberam 100 µg GnRH no momento da IATF. 2) Convencional: no dia 7, os dispositivos DIB foram removidos e 500 µg de d-CS e 0,5 mg de cipionato de estradiol foram administrados IM e tinta da cauda. Quarenta e oito horas após a remoção do DIB, as novilhas com a tinta removida foram DEAI. Aqueles que não apresentaram estro 54 h após a remoção do DIB foram FTAI. A prenhez foi diagnosticada por ultrassonografia 30 dias após a FTAI em todas as novilhas. A TP não diferiu entre os protocolos (44,28% e 42,48%, P = 0,673). Porém, em ambos os protocolos, maior TP foi observada em novilhas DEAI (P = 0,047). Concluindo a utilização do protocolo J-Synch gerou TP semelhantes ao protocolo convencional, permitindo sua utilização com a mesma eficácia. Além disso, a DEAI gerou melhores TP do que a FTAI, independentemente do protocolo utilizado.
The objective of the present work was to evaluate the effect of J-Synch protocol on pregnancy rate (PR) of heifers artificially inseminated after detected estrus (DEAI) or at fixed-time (FTAI). Two hundred ninety-three Braford heifers were used. On day 0, all animals received 2.0 mg of estradiol benzoate administered intramuscularly (IM) and an intravaginal progesterone-releasing device (DIB, 0.5 g). Heifers were assigned to the following group: 1) J-Synch: on day 6, DIB devices were removed and 500 µg of d-Cloprostenol sodium (d-CS) was administered IM and paint was applied on tail base (tail-paint). On day 9 (60 h after DIB removal), heifers with ≥ 40% of the tail-paint rubbed off were inseminated. Those not showing estrus by 72 h after DIB removal received 100 µg of GnRH at that time and were FTAI. 2) Conventional: on day 7, DIB devices were removed, and 500 µg of d-CS and 0.5 mg of estradiol cypionate were administered IM and tail-paint. Fortyeight hours after DIB removal, heifers with the tail-paint rubbed off were DEAI. Those not showing estrus by 54 h after DIB removal were FTAI. Pregnancy rate was diagnosed by ultrasonography 30 days after FTAI in all heifers and did not differ between protocols (44.28% and 42.48%, P = 0.673). In both protocols, higher PR was observed in DEAI heifers (P = 0.047). In conclusion, the use of the J-Synch protocol generated similar PR to the Conventional protocol, allowing its use with the same efficiency. Furthermore, the DEAI generated better PR than the FTAI, regardless of the protocol used.
Fil: Reineri, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Palma, Gustavo Adolfo. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Coria, Maria Sumampa. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina. Universidad Nacional de Santiago del Estero; Argentina
Fil: Perez, Rodrigo Punta. Universidad de Sevilla; España
Fil: Principi, Santiago. No especifíca;
https://purl.org/becyt/ford/4.4, GNRH, SYNCHRONIZATION PROTOCOLS, https://purl.org/becyt/ford/4, ESTRUS, TAIL-PAINT
https://purl.org/becyt/ford/4.4, GNRH, SYNCHRONIZATION PROTOCOLS, https://purl.org/becyt/ford/4, ESTRUS, TAIL-PAINT
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