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CONICET Digital
Article . 2013
License: CC BY NC SA
Data sources: CONICET Digital
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Hongos asociados con dos poblaciones de Acromyrmex lobicornis (Formicidae) de San Luis, Argentina

Authors: Lugo, Mónica Alejandra; Crespo, Esteban María; Cafaro, Matias Jose; Jofre, Laura Elizabeth;

Hongos asociados con dos poblaciones de Acromyrmex lobicornis (Formicidae) de San Luis, Argentina

Abstract

Los géneros Atta y Acromyrmex comprenden a las hormigas cortadoras o podadoras de hojas de la tribu Attini, único grupo de hormigas que presenta una dependencia obligada con simbiontes fúngicos como fuente de alimento. Los fragmentos de plantas recolectados por estas hormigas son utilizados para cultivar al simbionte fúngico, el que origina gongilidios de los cuales se alimentan las larvas y la reina de la colonia. El objetivo de este trabajo fue aislar y caracterizar genómica y taxonómicamente los hongos cultivados por dos poblaciones de Acromyrmex lobicornis de la provincia de San Luis. Para ello, se recolectaron muestras del simbionte de las cámaras de cultivo más superficiales de los nidos y se cultivaron en los medios de extracto de malta (EM) y agar papa dextrosa (APD). El simbionte fúngico se identificó como Leucoagaricus gongylophorus (Möller) Singer (Holobasidiomycetes, Agaricales) por sus características morfo-anatómicas en cultivo, las secuencias del gen 5.8S ribosomal y de los espaciadores ITS1 e ITS2. El diámetro de las colonias de los simbiontes fúngicos aislados fue mayor en EM; además, mostraron crecimiento diferencial entre las poblaciones de hormigas de las que fueron aislados. Se discuten posibles implicancias nutricionales y aspectos relacionados con la distribución de los simbiontes aislados.

Fungi associated with two populations of Acromyrmex lobicornis (Formicidae) from San Luis, Argentina. The ant genera Atta and Acromyrmex (Tribe Attini) include the mowers or trimmers of leaves called leaf-cutting ants, which are the only ants that show an obligate dependence of fungal symbionts as a food source. Fragments of plants collected by these ants are used to grow the fungal symbionts, which produce gongylidia for the larvae and queen of the colony to feed on. The aim of this study was to isolate and characterize both, genetically and taxonomically the fungi cultured by two populations of Acromyrmex lobicornis from San Luis province. Samples were collected from the most superficial chambers of the nests and fungal isolates were cultured in malt extract (ME) and potato dextrose agar (PDA) media. The fungal symbiont associated to A. lobicornis nests was identified as Leucoagaricus gongylophorus (Möller) Singer (Holobasidiomycetes, Agaricales) through its morpho-anatomic characteristics and sequencing of ITS1, ITS2 and 5.8S ribosomal gene. Fungal growth in ME was higher than in PDA, and symbiont isolates showed differential growth rates depending on ant populations from where they originated. Fungal symbiont nutritional features and distributional aspects are discussed.

Fil: Lugo, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - San Luis. Instituto Multidiciplinario de Investigación Biológica de San Luis; Argentina;

Fil: Jofre, Laura Elizabeth. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia; Argentina;

Fil: Crespo, Esteban María. Diversidad Vegetal I. Universidad Nacional de San Luis; Argentina;

Fil: Cafaro, Matias Jose. Universidad de Puerto Rico; Puerto Rico;

Country
Argentina
Keywords

ACROMYRMEX LOBICORNIS, CENTRAL ARGENTINA, LEAF-CUTTER ANTS, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1, FUNGAL SYMBIONT, LEUCOAGARICUS GONGYLOPHORUS

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green