The TrollTransect2025 cruise (TT25) was the annual cargo supply mission to Troll Station, Antarctica. The cruise was conducted aboard the M/V Silver Mary, travelling from Cape Town to the Antarctic ice shelf edge near 70°S, 3°E carrying equipment and supplies for the research station. The ship left Cape Town on December 28, 2024 and returned to the same port on February 1, 2025. Since 2020, the Norwegian Polar Institute (NPI) has taken advantage of the opportunity given by annual cargo missions to Troll research station to conduct a program of scientific observations in order to better understand the most important physical, biogeochemical and biological processes in King Haakon VII Sea of the Southern Ocean. The cruise objectives alternate every other year; for the TT25 cruise, priorities included the turnover of the three Multidisciplinary Ocean Moored Observatory DML ocean moorings on the Antarctic shelf break and multidisciplinary hydrographic surveying across the continental slope. This report details the scientific part of the cruise.