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Indian Journal of Experimental Biology
Article . 2001
Data sources: Europe PubMed Central
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Partial characterization of serum immunoglobulins of Oreochromis mossambicus.

descriptionPublicationkeyboard_double_arrow_right Article 25 Jan 2001 English Journal:Indian journal of experimental biology, volume 38 (issn: 0019-5189, Copyright policy )
Authors: P B, Rajavarthini; R I, Arunkumar; R D, Michael;

pmid: 11116524

Partial characterization of serum immunoglobulins of Oreochromis mossambicus.

Abstract

Serum immunoglobulins of O. mossambicus were purified using chromatography methods--CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each.

Related Organizations
Keywords

Immunoglobulins, Serum Albumin, Bovine, Hemagglutination Tests, Blood Protein Electrophoresis, Chromatography, Ion Exchange, Molecular Weight, Precipitins, Immunoglobulin M, Chromatography, Gel, Animals, Cattle, Electrophoresis, Polyacrylamide Gel, Immunization, Rabbits, Immunoelectrophoresis, Tilapia

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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