Sulfated cyclodextrins (SCDs) belong to the most popular chiral selectors in capillary electrophoresis [1, 2]. SCDs are available as mixtures of randomly highly sulfated species (HSCDs) or as well defined single isomer compounds (SI-CDs). For the randomly HS-CDs, only an average degree of substitution (DS) is estimated but the isomeric heterogeneity and the charge distribution are not known. In the SI-CDs, the sulfatation positions and the DS are well specified. However, due to close vicinity of the sulfate groups in both types of SCDs, counterion condensation can occur in their molecules and their actual effective charge can be lower than number of sulfate groups. For that reason, the aim of this work was to estimate the degree of charge reduction in both types of SCDs using the procedure based on separation of SCDs and reference compounds by capillary isotachophoresis (CITP) and zone electrophoresis (CZE). \n