This study focuses on the phosphorus (P) release from sediment of inflow part of Vranov reservoir under different availability of terminal electron acceptors, mainly dissolved O2 and NO3-. To understand the cycling of P in sediments, sediment cores were incubated for five weeks in three parallel variations that simulated: i) anoxia with depleted NO3-, ii) O2 saturated water column and iii) O2 saturated water column in the presence of high concentrations of NO3-. Evaluating the release of P and the impact of Iron (Fe) and its metabolism in sediment under the different conditions, water above the sediments was monitored, porewater composition and different P fractions in the sediment were analyzed.